张俊伟, 郭盘江, 陈冠吉. 人参果组织培养技术研究[J]. 西南林业大学学报, 2003, 23(2): 21-22. DOI: 10.11929/j.issn.2095-1914.2003.02.007
引用本文: 张俊伟, 郭盘江, 陈冠吉. 人参果组织培养技术研究[J]. 西南林业大学学报, 2003, 23(2): 21-22. DOI: 10.11929/j.issn.2095-1914.2003.02.007
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ZHANG Jun-wei, GUO Pan-jiang, CHEN Guan-ji. A Study on Culture Technology of Tissue of Solanum muricatum[J]. Journal of Southwest Forestry University, 2003, 23(2): 21-22. DOI: 10.11929/j.issn.2095-1914.2003.02.007
Citation: ZHANG Jun-wei, GUO Pan-jiang, CHEN Guan-ji. A Study on Culture Technology of Tissue of Solanum muricatum[J]. Journal of Southwest Forestry University, 2003, 23(2): 21-22. DOI: 10.11929/j.issn.2095-1914.2003.02.007
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人参果组织培养技术研究

A Study on Culture Technology of Tissue of Solanum muricatum

    摘要
  • 摘要: 以人参果的幼嫩茎段和叶片作外植体,在MS基本培养基附加不同浓度6-BA+IAA,6-BA+NAA和KT+2,4D组成的固体培养基中进行培养。结果显示:MS+6-BA 0.07mg/L+IAA 0.03mg/L,MS+6-BA 0.70mg/L+IAA 0.03mg/L,MS+6-BA 0.70mg/L+IAA 0.05mg/L,MS+6-BA 1.00mg/L+IAA 0.05mg/L 4种培养基中的材料愈伤组织长势比较好,分化明显。经继代培养,丛生芽大量形成,丛生苗根系发达。其他培养基则停留在愈伤组织阶段或只长出极少数短的根。

     

    Abstract: The delicate stem cuttings of Solanum muricatum.were inoculated on MS base medium supplied with different concentrations of 6-BA and IAA.The results showed that,the differentiation of callus on the MS+6-BA 0.07mg/L+IAA 0.03 mg/L,MS+6-BA 0.70 mg/L+IAA 0.03 mg/L,MS+6-BA 0.70 mg/L+IAA 0.05 mg/L,MS+6-BA 1.00 mg/L+IAA 0.05 mg/L were obvious.By continuing culture,the number of roots increased and grew well.The other stem cuttings were difficult to induced to callus or few roots were induced.

     

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