熊智, 张成刚, 张忠泽, 姜成林. 交叉接种后固氮放线菌基因稳定性研究[J]. 西南林业大学学报, 2003, 23(3): 1-4. DOI: 10.11929/j.issn.2095-1914.2003.03.001
引用本文: 熊智, 张成刚, 张忠泽, 姜成林. 交叉接种后固氮放线菌基因稳定性研究[J]. 西南林业大学学报, 2003, 23(3): 1-4. DOI: 10.11929/j.issn.2095-1914.2003.03.001
shu
XIONG Zhi, ZHANG Cheng-gang, ZHANG Zhong-Ze, JIANG Cheng-Lin. The Genetic Stability of Frankia after Cross Inoculation[J]. Journal of Southwest Forestry University, 2003, 23(3): 1-4. DOI: 10.11929/j.issn.2095-1914.2003.03.001
Citation: XIONG Zhi, ZHANG Cheng-gang, ZHANG Zhong-Ze, JIANG Cheng-Lin. The Genetic Stability of Frankia after Cross Inoculation[J]. Journal of Southwest Forestry University, 2003, 23(3): 1-4. DOI: 10.11929/j.issn.2095-1914.2003.03.001
shu

交叉接种后固氮放线菌基因稳定性研究

The Genetic Stability of Frankia after Cross Inoculation

    摘要
  • 摘要: 放线菌Frankia与放线菌根植物在根瘤中共生的现象发生在8科24属被子植物上。系统发育分析表明:Frankia可分为4个族,其菌株对寄主植物有明显的偏好性。用大理旱冬瓜根瘤接种昆明和武定旱冬瓜幼苗,收集幼苗上的根瘤,再对幼苗接种数次。用武定旱冬瓜和川滇桤木幼苗做同样实验,最后对Frankia的16SrRNA基因进行ARDRA分析,发现接种幼苗根瘤中Frankia与接种根瘤中Frankia产生相同的16SrRNA基因ARDRA带型。

     

    Abstract: The Frankia-actinorhizal plant root symbiosis occurs in 8 families of angiosperms.Phylogenetic analyses show that Frankia can be defined as 4 chades and a close relationship appears between the hosts and Frankia clades.Though there have been many studies on cross inoculation of Frankia,no reports showed gene variation after inocuation.Nodules of A.nepalensis from Dali were inoculated to Alnus nepalensis seedlings from Kunming and Wuding,the nodules were collected and reinoculated to new seedlings for several times.seedlings nodules of A.nepalensis and A.ferdinandi-coburgii from Wuding were done the same inoculation in the experiment.After inoculation,Frankia 16 S rRNA genes in seedlings nodules were amplified,and analyzed with ARDRA technique.The results show that the ARDRA band pattern of Frankia 16 S rRNA gene in inoculating nodules was the same as in the original seedlings nodules.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回