刘小烛, 铃木義胜, 山口勇. 银杏细胞对抗生素的耐受性研究[J]. 西南林业大学学报, 2004, 24(2): 1-2,6. DOI: 10.11929/j.issn.2095-1914.2004.02.001
引用本文: 刘小烛, 铃木義胜, 山口勇. 银杏细胞对抗生素的耐受性研究[J]. 西南林业大学学报, 2004, 24(2): 1-2,6. DOI: 10.11929/j.issn.2095-1914.2004.02.001
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LIU Xiao-zhu, Yoshi Cuzuki, Isamu Yamaguchi. Tolerance of Ginkgo biloba Callus Cells with Antibiotics[J]. Journal of Southwest Forestry University, 2004, 24(2): 1-2,6. DOI: 10.11929/j.issn.2095-1914.2004.02.001
Citation: LIU Xiao-zhu, Yoshi Cuzuki, Isamu Yamaguchi. Tolerance of Ginkgo biloba Callus Cells with Antibiotics[J]. Journal of Southwest Forestry University, 2004, 24(2): 1-2,6. DOI: 10.11929/j.issn.2095-1914.2004.02.001
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银杏细胞对抗生素的耐受性研究

Tolerance of Ginkgo biloba Callus Cells with Antibiotics

    摘要
  • 摘要: 用MS培养基诱导银杏胚产生愈伤组织.分别在MS培养基中加入不同浓度的卡那霉素、潮霉素和杀稻瘟菌素,将银杏愈伤组织转接到含抗菌素的培养基上继续培养11d之后得到以下结果:MS培养基中含潮霉素25mg/L时,银杏愈伤组织变成褐色,停止生长;MS培养基中含同样浓度的杀稻瘟菌素时,银杏愈伤组织变成浅褐色生长变慢;MS培养基中含同样浓度的卡那霉素时,银杏愈伤组织生长良好.银杏细胞对潮霉素的耐受性很弱,可以作为筛选转基因银杏树种的抗生素使用.

     

    Abstract: The results obtained by having transplanted the callus of Ginkgo biloba produced from embryo on normal MS medium into mediums with different antibiotics for 11 days were as follows:(1)the callus cells turned brown and stopped growth on MS medium with 25 mg/L of hygromycin;(2)the callus cells turned light brown and partially stopped growth onMS medium with 25 mg/L blasticedin;(3)the callus cells of Ginkgo biloba grew well onMS medium containing 25 mg/L kanamycin.It showed that the callus cells of Ginkgo biloba were susceptible to hygromycin, and hygromycin could be used as antibiotics for screening of gene transferred varieties of Ginkgo biloba.

     

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