Abstract: Zoo construction is one of the principal manners for ex situ conservation of animals.The fundamental circumstances of feeding environment, management and breeding facilities of animals were described by analyzing Kunming Zoo as an example.Both successful and failed experiences in ex situ conservation of animals in zoos were summarized.Requirements of habitat environment for different animal species through imitating the original environments to provide the animals with appropriate shelters and to prevent diseases from spreading by reasonable layout and rational animal densities were discussed.It was suggested that isolated breeding divisions should be created, breeding populations of local species and rare animals should be established, and development mechanism of breeding behaviors of the animals should be studied.It was also suggested that the animals should be trained for feeding, mating, defending and communicating under captive circumstances.