Abstract: Ginkgo biloba is a tall tree species with luxuriant leaves and huge biomass.If a purpose gene could be transferred into this tree species and if the transferred gene could express and be heritable steadily in callus cells of Ginkgo biloba, the production of plant species with the transferred purpose gene would be high.To meet the demands of gene transfer of an objective gene, a suitable vector should be screened to carry the extraneous objective gene into the callus cell of Ginkgo biloba.Plasmid pBI221 carrying GUS gene linked with the CaM35S promoter and the NOS terminator was shot into callus cells of Ginkgo biloba by the particle gun bombardments, and the activity of GUS gene was examined by X -Gluc substrate.It was showed by the results that GUS gene was successfully transferred into callus cells of Ginkgo biloba, and the transferred GUS gene was expressed in the callus cells of Ginkgo Biloba, however, the transferred gene did not stably exist in the callus cells of Ginkgo biloba