Abstract: The entomopathogenic fungal isolate (Paecilomyces farinosus)sw 03085 was cultured in submerged condition at 25℃using 9 hydrocarbons, 7 nitrogenous compounds and inorganic matters.Based on the production of dry mycelia in incubation time, four hydrocarbons (corn flour, saccharose, maltose, dextrose)were selected for using in mass liquid ferment, and in 72 h dry mycelia production could reach 7.33~8.04 mg/mL.The dry mycelia biomass in Beef peptone media could reach 8.87 mg/mL in 72 h that was 3.5~6.0 times than the production in other nitrogenous substance media.Among several experimental inorganic matters, KH2PO4was the most fitful inorganic salt and the mycelia biomass could be 7.62mg/mL in 72 h.The experimental result also indicated FeSO4 and CaSO4 benefit to the production of nodal spores of the fungus and spore concentration could be 10⁸/mL