Abstract: An orthogonal test with 3 factors in 4 treatment levels was carried out to study the influence of different factors including pretreatment, plant growth regulator and culture medium on the rooting from varied-aged needle-leaved bundles of Pinus kesiya var.langbianensis. It was indicated that the leading factor affecting the rooting rate of 2 or 3-year-old needle-leaved bundles was the culture medium, and the major influencing factor upon the rooting rate of 4-year-old needle-leaved bundles was pretreatment.It was also showed by the test results that both of the rooting rate and rooting speed dropped along with the increment of ages of the needle-leaved bundles.It was proven by the test that the highest rooting rate was obtained by 3-year-old needle-leaved bundles cultured by No.9 medium with 75 mg/kg of H₃BO₄, 20 mg/kg of NH₄NO₃ and 20 mg/kg of vitamin B₁.