陈礼光, 高培军, 谢安强, 郑郁善, 刘学琴, 林秀桂. 杉木种子超干燥和超低温贮藏研究I.种子质量贮藏效果[J]. 西南林业大学学报, 2005, 25(4): 113-117. DOI: 10.11929/j.issn.2095-1914.2005.04.025
引用本文: 陈礼光, 高培军, 谢安强, 郑郁善, 刘学琴, 林秀桂. 杉木种子超干燥和超低温贮藏研究I.种子质量贮藏效果[J]. 西南林业大学学报, 2005, 25(4): 113-117. DOI: 10.11929/j.issn.2095-1914.2005.04.025
CHEN Li-guang, GAO Pei-jun, XIE An-qiang, ZHEN Yu-shan, LIU Xue-qin, LIN Xiu-gui. Study on Ultra-dried Seed Storage and Cryopreservation of Cunninghamia lanceolata I.Storage Effects on Seed Qualities[J]. Journal of Southwest Forestry University, 2005, 25(4): 113-117. DOI: 10.11929/j.issn.2095-1914.2005.04.025
Citation: CHEN Li-guang, GAO Pei-jun, XIE An-qiang, ZHEN Yu-shan, LIU Xue-qin, LIN Xiu-gui. Study on Ultra-dried Seed Storage and Cryopreservation of Cunninghamia lanceolata I.Storage Effects on Seed Qualities[J]. Journal of Southwest Forestry University, 2005, 25(4): 113-117. DOI: 10.11929/j.issn.2095-1914.2005.04.025

杉木种子超干燥和超低温贮藏研究I.种子质量贮藏效果

Study on Ultra-dried Seed Storage and Cryopreservation of Cunninghamia lanceolata I.Storage Effects on Seed Qualities

  • 摘要: 采用热恒温干燥法,研究不同脱水干燥温度对杉木种子质量的影响,通过超干燥处理后的种子贮藏1年、超低温保存、防冻剂预处理超低温保存不同含水量的杉木种子,分析其发芽率和种子活力,研究杉木种子贮藏后的质量变化.结果表明:50℃是杉木种子脱水的适宜温度,种子发芽率和活力指数最高;3%~5%的含水量是杉木种子超干燥保存的最适含水量,贮藏1年与常规低温贮藏效果没有显著差异.超低温保存表明,9%含水量的种子贮藏效果要比超干燥处理后贮藏好,可用于杉木种子的中长期保存.防冻剂超低温保存受防冻剂种类和浓度影响显著,尤其是二甲亚砜的浓度,冷冻方式和解冻方式的影响作用则被防冻剂削弱。

     

    Abstract: The influence of different desiccation temperature on the qualities of Cunninghamia lanceolata seeds was studied by thermal constant temperature desiccation method.The seed quality was tested by germination and vitality experiments for Cunninghamia lanceolata seeds after ultra-dried storage for 1 year and then treated by cryopreservation or by cryopreservation after being pretreated by cryoprotectants with different water contents.It was concluded that the rational desiccation temperature was 50℃, the treated seeds had the highest germination rate and vitality index.3%~5% moisture contents of Cunninghamia lanceolata seeds were the most suitable for ultra-dried storage, and there was no significant difference in tested indexes within this scale of moisture contents after having been stored for 1 year by cryopreservation or by ordinary low-temperature storage.It was showed that the storage effects by cryopreservation was better than ultra-dried storage when the water content was 9%, which might be a good way for long term germplasm storage for Cunninghamia lanceolata.The types and concentrations of cryoprotectants, especially DMSO concentration, would significantly influence on the storage effect by cryopreservation. It was showed that the effects of freezing and thawing method were weakened by cryoprotectants.

     

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