Abstract: By makusing use of apical buds or stem sections as the explants, the tissue culture of the stump shoots of Eucalytus urophylla × E. camalduiensis was experimented with different fundamental media, different types and different concentration levels of plant growth regulators. It was showed by the results that the optimal medium for explant induction was the improved MS+6-BA 0.3 mg/L+IBA 0.5 mg/L, and the induction rate reached 71.2%. The best medium for bud multiplication was the improved MS+6-BA 0.4mg/L+NAA 0.6mg/L and the best medium for rooting was the improved MS+IBA 1.0 mg/L, whose rooting rate reached 98%. Red-heart soil was the optimal substrate material for transplanting of the nurtured young plant.