Abstract: The competitive capacity is the springhead for the clusters to acquire sustainable competition advantages. The competitiveness of nature reserve ecotourism of Yunnan Province is unique not only with specific nature resource characteristics but also with the harmonious coordination and unity of relevant ecotourism enterprises in the province. Acquiring of the cluster competitive capacity of nature reserve ecotourism in Yunnan Province relies on not only internal conformity of the relevant resources but also relies on integrating the corresponding external resources by building efficient supply chain system. The factors that influence on the cluster competitive capacity were analyzed. It was proposed that the connotative heterogeneity of the supply chain should be paid great attention, and the promotion of the cluster competitive capacity of nature reserve ecotourism of Yunnan Province would be realized by building effective supply chain interface.