陈剑英. 油杉属植物过氧化物同工酶研究[J]. 西南林业大学学报, 2007, 27(2): 46-49. DOI: 10.11929/j.issn.2095-1914.2007.02.012
引用本文: 陈剑英. 油杉属植物过氧化物同工酶研究[J]. 西南林业大学学报, 2007, 27(2): 46-49. DOI: 10.11929/j.issn.2095-1914.2007.02.012
On Peroxidase Isoenzymes of Plants in Genus of Keteleeria[J]. Journal of Southwest Forestry University, 2007, 27(2): 46-49. DOI: 10.11929/j.issn.2095-1914.2007.02.012
Citation: On Peroxidase Isoenzymes of Plants in Genus of Keteleeria[J]. Journal of Southwest Forestry University, 2007, 27(2): 46-49. DOI: 10.11929/j.issn.2095-1914.2007.02.012

油杉属植物过氧化物同工酶研究

On Peroxidase Isoenzymes of Plants in Genus of Keteleeria

  • 摘要: 用Disc聚丙酰胺凝胶电泳分析了油杉属8个种的过氧化物同工酶.结果表明:属内种间具有明显的酶谱差异,每个种都有其特征酶谱.用数量分类中的系统聚类方法对油杉属8个种酶谱的相似程度进行了研究,结果把8个种分为3个类群,6个种,归并了2个种.其中:油杉、江南油杉为一类群,铁坚油杉、黄枝油杉为一类群,云南油杉、旱地油杉为一类群,铁坚油杉与青岩油杉归并为同一物种,云南油杉与蓑衣油杉归并为同一物种,推断旱地油杉为云南油杉的生态变种,从分子水平上论证了油杉属各种间的亲缘关系.

     

    Abstract: Peroxidase isoenzymes of 8 species in genus of Keteleeria were analyzed by means of Disc polyacrylamide gel electrophoresis. It was showed that each species had its specific zymogram and the differences in zymograms among the species were obvious. The similarity of zymograms of the 8 species was studied by systematic clustering method applied to quantitative analysis. According to the zymograms, the 8 species might be classified into 3 groups and 6 species, by which 2 pairs of 2 species were merged respectively into 2 species. GroupⅠincluded Keteleeria fortunei and Keteleeria cyclolepis. Group Ⅱ contained Keteleeria davidiana and Keteleeria calcarea. The third group comprised Keteleeria evelyniana and Keteleeria xerophila. It was proposed that Keteleeria davidiana var. chien-peii should be merged with Keteleeria davidiana, and Keteleeria evelyniana var. pendula should be merged with Keteleeria evelyniana. It was deduced that Keteleeria xerophila might be an ecotype of Keteleeria evelyniana. The results from peroxidase isoenzyme experiment verified at molecular level the consanquineous relations among the species in genus of Keteleeria.

     

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