Abstract: A tissue culture experiment with Primula obconica was carried out and tender leaves were used as the initial explant to induce differentiation. The result revealed that the induced tender leaves of Primula obconica would form callus first and then differentiate into buds. The comparatively suitable medium for young leaf induction was MS+6-BA 2.0 mg/L+NAA 1.0 mg/L, on which the average inducing rate reached 42.11%. The better medium for successive culture was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L, on which the average differentiation coefficient for effective buds reached 4.27, and the mean height of young shoots was 1.5cm. Ordinary MS medium was the best for rooting, on which the average number of roots occurred was 8.6, with the average root length as 3.5 cm, and the average shoot height as 5.0 cm. The roots induced by the ordinary medium were strong enough for transplanting. The material for Primula obconica transplanting was the combination of river mud and perlite (proportion 7:3), the average survival rate could reach 98.1% in the experiments.