湿地松器官性愈伤组织诱导与增殖

Induction and Multiplication Culture of Organogenetic Callus of Pinus caribaea

摘要: 以湿地松成熟合子胚、胚苗顶芽、下胚轴、胚根为外植体,选用DCR,LP,TX 3种培养基,与不同浓度NAA,2,4-D及6-BA组合,研究其器官性愈伤组织诱导、增殖培养的最适条件.结果表明:成熟合子胚、胚苗顶芽是诱导器官性愈伤组织理想的外植体,最佳诱导培养基为TX+BA 0.5 mg/L+NAA 2.0 mg/L+30 g/L蔗糖,诱导率达50%以上;最佳增殖培养基为TX+BA 0.3 mg/L+NAA 1.0 mg/L+CH 400 mg/L+30 g/L蔗糖,继代周期25~30 d,增殖率达3.46.

Abstract: The optimum conditions for organogenetic callus induction and multiplication culture were studied for Pinus caribaea by taking the mature zygotic embryos,apical buds of embryo sprouts,hypocotyls and radicle as explants.Three media DCR,LP,TX with different concentration of NAA,2,4-D,6-BA plant growth regulators were applied.The results showed that the mature zygotic embryos and apical buds of the embryo sprouts were best explants for induction of organogenetic callus,and the best induction medium was TX+BA 0.5 mg/L+NAA 2.0 mg/L+30 g/L sucrose,the induction rate reached 50%.The best medium for multiplication culture was TX+BA 0.3 mg/L+NAA 1.0 mg/L+CH 400 mg/L+30 g/L sucrose,the successive transfer cycle was 25~30 d,and the multiplication rate was 3.46.