Abstract: Various plant growth regulator combinations and culture medium types were examined to discussed their effects on startup,proliferation and rooting culture of Cerasus serrulata var.lannesiana‘Mollis’and Cerasus serrulata varlannesiana‘Zuihong’by using stem with bud as explants.The result showed that the medium of MS+10mg/L 6-BA+01mg/L NAA was the most effective for two cultivars to startup culture.In the medium,axillary bud germination rate of‘Mollis’and,‘Zuihong’was 73% and 80%,respectively.Optimal shoot proliferation was obtained in medium of WPM+10mg/L KT+01mg/L IBA. Buds were pretreatment in rooting medium of 1/2 WPM+10mg/L IBA+05mg/L NAA which was added hormone for 4d,and then transfer to a hormonefree medium for 24 days,the rooting percentage of‘Zuihong’and‘Mollis’were 93% and 90%,respectively.The rooted plantlets were transplanted in mixed medium which was compose of perlite,peat and vermiculite,the survival rate was up to 80% after hardening for 30 d.