Abstract: after unified cut apical buds for 2yearold Pinus yunnanensis seedlings, the L9（34） orthogonal design was applied to implement the experiment of fertilization, praying BY2 and IBA solution on needles, which purpose was to detect the effects of factors and their levels with treatment combinations (TC) on cutting yield, etc. Mean numbers of sprouting buds and new branches were 132-187 bud/plant and 44-97 branch/plant, respectively, when the experiment was carried out for 90 days. After 180 days, mean cutting outputs and the ratios of cutting/branch between the TCs were 26-59 cutting/plant and 13%-522%, respectively, which main factor of influencing these two parameters was BY2, with the optimal theoretical TC of fertilizing organic fertilizer for 005 kg/plant and spraying BY2 solution on the needles for the 40×107 CFU/mL. Organic fertilizer facilitated sprouting buds and increased the cutting yield; the IBA improved sprouting buds and branch growth, while which limited the buds to form the branches; and BY2 could increased cutting yield. Sound seedlings of presenting large basal diameters with non grass stage are the pivotal element of increasing cutting output of P. yunnanensis; simultaneously, based on the condition of sufficient growth nutrients for 2yearold seedlings, spraying 40×107 CFU/mL of the BY2 solution on the needle could be facilitated cutting yield as well.