Abstract: The change of microbial community characteristics in the different rhizosphere zero of hybrid poplars（Populus deltoids×nigra,DN34） under atrazine stress were investigated by rhizobox and Biolog system in a green house experiment，with sublimed sulfur and calcium hydroxide as soil pH regulators. Data from measure by Biolog ECO plate demonstrated that the value of average well color development (AWCD) and the carbon source utilization of microorganism in soil amending soil pH regulators was promoter than CK during the initial 30 days; While the results from the 90 days indicated that the activity and the carbon source utilization of soil microbe in acid regulator treatment began to fall down, even lower than that of poplar implant treatment, then the alkaline treatment still stimulated and promoted the activity and the carbon source utilization ability of soil microbe. Overall, the activity and community structure of soil microbe in rhizosphere regional of atrazinecontaminated soil did not show steady regularity during the phytoremediation process by using hybrid poplars（Populus deltoids×nigra，DN34）. For the reason that the Biolog ECO plate, which only had 31 kinds of carbon sources, could not really indicate the detail information about the function diversity of community structure of soil microbe. So its essential for us to take more sophisticated analysis tools for further study.