廖丹, 程江波, 聂风琴, 李明, 林道哲, 符文英. 海巴戟ISSR遗传多样性分析[J]. 西南林业大学学报, 2016, 36(2): 72-77. DOI: 10.11929/j.issn.2095-1914.2016.02.012
引用本文: 廖丹, 程江波, 聂风琴, 李明, 林道哲, 符文英. 海巴戟ISSR遗传多样性分析[J]. 西南林业大学学报, 2016, 36(2): 72-77. DOI: 10.11929/j.issn.2095-1914.2016.02.012
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Liao Dan, Cheng Jiangbo, Nie Fengqin, Li Ming, Lin Daozhe, Fu Wenying. Genetic Diversity Analysis of Morinda citrifolia by ISSR Markers[J]. Journal of Southwest Forestry University, 2016, 36(2): 72-77. DOI: 10.11929/j.issn.2095-1914.2016.02.012
Citation: Liao Dan, Cheng Jiangbo, Nie Fengqin, Li Ming, Lin Daozhe, Fu Wenying. Genetic Diversity Analysis of Morinda citrifolia by ISSR Markers[J]. Journal of Southwest Forestry University, 2016, 36(2): 72-77. DOI: 10.11929/j.issn.2095-1914.2016.02.012
shu

海巴戟ISSR遗传多样性分析

Genetic Diversity Analysis of Morinda citrifolia by ISSR Markers

    摘要
  • 摘要: 利用ISSR分子标记对202份海巴戟种质材料进行遗传多样性和聚类分析。结果表明：13条ISSR引物共扩增出65个位点，每个引物平均扩增出5个位点，其中多态性位点63个，占总数的9692%。在相似系数为063时，202份海巴戟种质分为5个类，表现出一定的地域分布规律。检测到有效等位基因数 Ne=16042，基因多样性 H=03488 ，Shannon′s信息指数 I=05206，说明202份海巴戟种质间存在着较高的遗传多样性。

     

    Abstract: The genetic diversity and cluster analysis of the 202 Morinda citrifolia germplasm resources were analyzed by using ISSR molecular markers. The results showed that 13 ISSR primers which amplified 65 loci, meaned the average of each primer could amplified five loci. Among them，63 bands （96.92%）were polymorphic. Cluster analysis result indicated that 202 germplasm resources could be clustered into five groups when the genetic similarity coefficient was 0.63， revealedthe characteristics of geographical distribution. The average of Shannon information index (I) , Neis genetic diversity (H) and effective number of alleles (Ne) was 0.5206, 0.3488 and 1.6042, respectively, which indicated there was highly genetic diversity in these 202 Morinda citrifolia resource .

     

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