Abstract: In this study, Qiongzhuea tumidinoda seedlings were chosen as the research objects. The effects on annual container seedling growth with 3 test factors including different container material, different container specification and different amount of slow release base fertilizer were systematic analyzed by orthogonal design method. The results showed that the light matrix container seedling with the treatment of 10cm × 14cm nonwoven bags and 2kg/m3 slow release fertilizer. Stem diameter and seedlings height were 033cm and 4050cm and 6190% and 2376% higher than the worst one, respectively. Tiller number was 820, 9523% above the worst treatment one. The root length and the number of first order roots were 2106cm and 2880, respectively. The seedlings biomass was 1501g, and the allocation ratio of root biomass was significantly increased. Nitrogen content and Chlorophyll content were 294mg/g and 3338mg/g, respectively. It was the highest seedling quality index. Accordingly, the treatment in this study was the best choice for annual seedlings of Qiongzhuea tumidinoda under light matrix container nursery.