基于高效液相色谱法对不同提取条件下竹叶
黄酮中牡荆苷含量的分析

基于高效液相色谱法对不同提取条件下竹叶黄酮中牡荆苷含量的分析

摘要: 用高效液相色谱对竹叶黄酮中牡荆素进行分析，并对其含量进行了测定，同时研究了提取溶剂比、料液比和提取时间对牡荆素含量的影响。结果表明：最佳的提取条件为，提取溶剂比为70%乙醇水，料液比为1∶20，提取时间为20h。采用牡荆苷作为外标物，建立牡荆苷标准曲线方程：m=4725 × 10-7A + 00425 （R2=0999）。在最佳的提取条件下，采用大孔树脂对提取物进行纯化，对竹叶黄酮中牡荆素的含量进行测定，灰金竹中牡荆素的含量为 (101% ± 007%)。

Abstract: We studied the Vitexin in flavonoids of bamboo leaves and measured the content with HPLC external standard method, simultaneously investigated the influences of extraction solvent ratio, solid to liquid ratio, and extraction time on the content of Vitexin in flavonoids of bamboo leaves. The results showed that the optimum extraction conditions were: extraction solvent ratio was 70% ethanolwater, solidliquid ratio was 1∶20, extraction time was 20h. We established the standard curve equation m=4725 × 10-7A + 00425 (R2=0999), using Vitexin as external standard substance and purifying extract with Macroporous resin. Under the optimum extraction conditions, the Vitexin content in Phyllostachys nigra var. henonis Stapf ex Rendle was (101% ± 007%).