Abstract: Ganoderma lobatum (C₀) and its biochar (C₄₀₀) were evaluated on biosorption of Pb (Ⅱ) from aqueous solutions under different conditions including adsorbent doses, initial pH, contact time and initial Pb (Ⅱ) concentrations. The results showed the optimum conditions of adsorbent doses was 4 g/L (C₀) and 0.8 g/L (C₄₀₀), pH 7 and contact time of 480 min andthe maximum removal efficiencies of Pb (Ⅱ) for C₀ and C₄₀₀ were 97.72% and 97.60%, respectively. The isothermal adsorption model of Langmuir and Freundlich could well fitted the biosorption processes of C₀ and C₄₀₀ for Pb (Ⅱ) and their maximum sorption capacity were 90.90 mg/g and 163.93 mg/g, respectively. Kinetic model fitting indicated that the biosorption processes of C₀ and C₄₀₀ for Pb (Ⅱ) followed well pseudo second order kinetics. The sorption rate of Pb (Ⅱ) on C₀ was significantly higher than that on C₄₀₀, but sorption capacity of C₄₀₀ was greater than of C₀.