Abstract: Taken germinative rootstock of Polygonatum cyrtonema as the material. And the experiment was based on MS + NAA 1.0 mg/L+ KT 1.5 mg/L medium. It was investigated the complex effect that made by the concentration of the 5 kinds of macroelements in MS medium on the growth of germinative rootstock of P.cyrtonema and the 2 kinds of secondary metabolites include amylose and total saponins. The results indicated that in the most suitable medium for the growth of P.cyrtonema germinative rootstock, the concentration of NH₄NO₃ and KNO₃ was 1.5 times as strong as the standard concentration of MS medium. The concentration of KH₂PO₄, MgSO₄ and CaCl₂ was 2 times as strong as the standard concentration of MS medium. In the most suitable medium that synthesized by total anylose, the concentration of KH₂PO₄ was 2 times as strong as the standard concentration of MS medium. In addition, the other elements were all as strong as the standard concentration of MS medium. In the most suitable medium that synthesized by total saponins, the concentration of 5 kinds of macroelements were MS medium′s standard concentration.