龙亚芹, 段春芳, 刘杰, 彭海明, 申科, 王雪松, 石华忠, 罗梓文, 陈雷, 李荣福. 小粒咖啡褐斑病病原菌鉴定及田间抗病性研究[J]. 西南林业大学学报, 2017, 37(2): 152-157. DOI: 10.11929/j.issn.2095-1914.2017.02.025
引用本文: 龙亚芹, 段春芳, 刘杰, 彭海明, 申科, 王雪松, 石华忠, 罗梓文, 陈雷, 李荣福. 小粒咖啡褐斑病病原菌鉴定及田间抗病性研究[J]. 西南林业大学学报, 2017, 37(2): 152-157. DOI: 10.11929/j.issn.2095-1914.2017.02.025
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Yaqin Long, Chunfang Duan, Jie Liu, Haiming Peng, Ke Shen, Xuesong Wang, Huazhong Shi, Ziwen Luo, Lei Chen, Rongfu Li. Identification of Pathogen Causing Coffee Tree Brown Leaf Spot and Disease Resistance of Coffea arabica Varieties in Field[J]. Journal of Southwest Forestry University, 2017, 37(2): 152-157. DOI: 10.11929/j.issn.2095-1914.2017.02.025
Citation: Yaqin Long, Chunfang Duan, Jie Liu, Haiming Peng, Ke Shen, Xuesong Wang, Huazhong Shi, Ziwen Luo, Lei Chen, Rongfu Li. Identification of Pathogen Causing Coffee Tree Brown Leaf Spot and Disease Resistance of Coffea arabica Varieties in Field[J]. Journal of Southwest Forestry University, 2017, 37(2): 152-157. DOI: 10.11929/j.issn.2095-1914.2017.02.025
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小粒咖啡褐斑病病原菌鉴定及田间抗病性研究

Identification of Pathogen Causing Coffee Tree Brown Leaf Spot and Disease Resistance of Coffea arabica Varieties in Field

    摘要
  • 摘要: 对咖啡褐斑病病原菌进行分离培养和致病性测定, 并结合形态学和分子鉴定, 确定其病原菌, 同时开展咖啡褐斑病田间发生情况和品种抗病性调查研究。结果表明:从不同地点采集的小粒咖啡病叶上分离出3个菌株, 其形态特征相同。选取从云南(CCYN05)和海南(CCHN05)样品上纯化到的2个菌株, 对其rDNA-ITS克隆测序分析, 2个菌株为同一致病菌。结合形态学特征, 将该病原菌鉴定为咖啡生尾孢。调查了5个小粒咖啡品种对褐斑病的田间抗性, 没有发现免疫品种和抗病品种, 5个品种抗病性由强至弱依次为卡杜埃瑞吉纳、卡杜拉、卡杜埃44、卡蒂膜、维拉萨奇。

     

    Abstract: Pathogen of coffee tree brown leaf spot were identified by means of isolation culture, pathogenicity test, and the combination of morphology and molecular biology identification.At the same time, the investigation of occurrence and varieties resistance on coffee brown spot disease were carried out.The results showed that morphological characteristics of 3 pathogenic strains from coffee leaf samples in different places were the same.CCYN05 and CCHN05 were purified, and 2 strains were the same pathogen by rDNA-ITS sequence alignment and analysis.And combined with morphology characteristics, the results revealed that this disease was caused by Cercospora coffeicola.The brown spot disease resistances of 5 Arabica coffee tree varieties were investigated in the field, there were no immune varieties and disease-resistant varieties.The disease resistances sequences were as following:Catuaí Regional, Caturra, Catuaí44, Catimor, Villa Sarchí.

     

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