响应面法优化超声辅助提取紫茎泽兰总黄酮工艺研究

Optimization of Ultrasonic-assisted Extraction of Flavonoids from Eupatorium adenophorum by Response Surface Methodology

摘要: 在单因素试验基础上，根据Box-Behnken设计原理，以得率为指标，应用响应曲面分析法对紫茎泽兰总黄酮的超声波辅助提取工艺进行优化，建立回归模型。结果表明：紫茎泽兰总黄酮的超声波辅助提取工艺优化回归方程为R₁=1.42 + 0.050A - 0.054B + 0.073C - 0.030AB +0.016BC - 0.013A²。紫茎泽兰最佳提取工艺条件为乙醇浓度53.66%，提取时间35 min，料液比1 : 30；此最佳工艺条件下总黄酮得率为1.612%。

Abstract: The optimized extraction conditions of flavonoids from Eupatorium adenophorum were studied by single experiment and response surface methodology. Based on single experiment and the Box-Behnken design principles, regression model of extraction of flavonoids from E.adenophorum were established. The results showed that regression equation was R₁=1.42 + 0.050A - 0.054B + 0.073C - 0.030AB + 0.016BC - 0.013A². The optimal extraction conditions were ethanol concentration of 53.66%, extraction time of 35 min and solid to liquid ratio of 1 : 30, the yield of flavonoids was 1.612%.