Abstract:
The transferability of 18
Pinus kesiya var.
langbianensis EST-SSR markers to related species was studied. The transferability percentages of selected primers to
P.massoniana,
P.densata,
P.tabuliformis and
P.yunnanensis were 88.89%. The percentage of polymorphism of 18 EST-SSR markers to
P.massoniana,
P.densata,
P.tabuliformis and
P.yunnanensis were 81.25%, 81.25%, 75.00% and 93.75%, respectively. These results indicated that it was feasible to develop SSR markers from
P.kesiya var.
langbianensis transcriptome sequences.
P.kesiya var.
langbianensis EST-SSR markers was highly transferable and polymorphic to its related species. These markers can be used to analyze the genetic relationship between the
P.kesiya var.
langbianensis and its related species.