Abstract: This research aimed at measuring Cinnamomum camphora seed size by the scanner which obtained standard graphics and ImageJ software which analyzed the seed size.Comparison and correlation analysis were based on vernier caliper method.Variation between the provenance and family level of seed size of 10 typical species of C.camphora was analyzed by this method.The results showed the correlation coefficient was larger than 0.986 8 which indicated the two methods were significantly positively related (P < 0.05).C.camphora seed size variation mainly from the provenance.The average diversity index was 1.05 which suggested small genetic variation level among populations, and less affected by climatic factors.Comparing with vernier caliper method, image determination was more rapid and accurate in determining C.camphora seed size.