Abstract: To study the feasibility of taking composted coffee seed-coat as seedling substrates for trees, 7 kinds of nursery substrates which were added inorganic nitrogen fertilizer, farmyard manure and microbial agents as amendment. Aiming at studying the influences of the coffee seed-coat container substrates on the quality of Pinus kesiya var. langbianensis seedlings. Results indicated that there were significantly impact among the indicators of seedling height, ground diameter, main root length, number of lateral root and biomass. Considering the QI and mineral nutrition in the seedlings, the 2 proportions could be applied preferably for culturing container seedlings. One substrate was mixed by V(coffee seed-coat) :V(cow dung)= 4:1, and another was mixed by enzyme 0.5 kg and super-phosphate 1.25 kg/m³ of coffee seed-coat.