杉木种鳞石蜡切片工艺优化研究

Process Optimization of Paraffin Section Method for Scale of Cunninghamia lanceolata

  • 摘要: 为精确观察杉木种鳞内部的细胞结构,经过对2种不同固定液、3种不同软化处理、4种不同脱水与透明处理、优化浸蜡时间和温度以及后续包埋、染色等工艺步骤对比,优化了杉木种鳞石蜡切片工艺。结果表明:杉木种鳞石蜡切片的优化工艺和方法为,FAA和FPA两种固定液固定效果相同,均能有效的固定材料;12 h的盐酸水溶液软化效果最佳;最佳脱水时间为每级2 h,透明时间为每级1 h。该优化工艺有效克服了蜡带不连续、切片不完整的缺陷,可获得清晰完整的杉木种鳞细胞结构图。

     

    Abstract: In order to accurately observe the cell internal structure of Cunninghamia lanceolata scales, the processes, which was summarized by comparing the results of different treatments including 2 different fixing solutions, 3 softening treatments, 4 dehydrant and transparent treatments, time and temperature of waxing, embedding and dyeing, were optimized. Results showed that the material could be both fixed effectively by the FAA and FPA solutions; the best softening process was the hydrochloric acid solution for 12 h; the best dehydration time was 2 h per level, and the best transparent time was 1 h per level. Clear and complete cell structure map of the scales of C.lanceolata could be obtained by using this method.

     

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