Abstract: By comparing the interactions of the generalist Prodenia litura, the specialist Gadirtha inexacta and Meloidogyne incognita on the growth and biomass of Sapium sebiferum, the interaction patterns between different natural enemies and the response strategies of plants were analyzed. The results showed that in the absence of Meloidogyne incognita, the two kinds of above-ground natural enemies significantly reduced the diameter of Sapium sebiferum, and the plant height with Gadirtha inexacta herbivory was significantly lower than the treatment without the presence of Meloidogune incognita. In the case of Meloidogune incognita, there was no significant difference in the effect of above-ground natural enemies on the diameter and plant height of Sapium sebiferum. Meloidogune incognita significantly increased the above-ground biomass and total biomass in the Gadirtha inexacta treatment group. Therefore, Meloidogune incognita and Gadirtha inexacta produced an antagonistic effect on each other, thus formed an additive effect on the growth and biomass of Sapium sebiferum. However, this pattern was not significant between Meloidogune incognita and Prodenia litura.