Abstract: Based on the data collected from sample plot of 4 hm², for analysing the relations between interspecific association and community succession, and discussing the effects of the negative density-dependent mechanism on the interspecific association, the overall associations and intra-and interspecific associations among tree species in the mid-montane humid evergreen broad-leaved forest in the Gaoligong Mountains in Yunnan Province were studied. The results showed that the overall associations of tree species in the plot showed significant positive, which suggesting that most of species can be in harmony in the community, and the community was in a relatively stable stage of succession. Most of species combinations among all the species, the dominant species and the species within the same family showed a independent associations, further indicated that most of species in the community had no apparent conflict in the utilization of resources, and their interdependence or intercompetition for the habitat requirements was weaker, more helpful to promote the development of their stability in the community. Intraspecific associations of most of species showed positive between the saplings and the medium trees, but independent between the saplings and the adults, which may be caused by the negative density-dependent effect.