Abstract: Taking the different types of land use in the typical karst area of Puzhehei where in the southeastern part of Yunnan Province as the study area. The contents of total calcium, all forms of calcium, total phosphorus, organic matter, pH and soil particle size (clay, silt and sand) of 5 kinds of land use types which including bare land, forest land, dry land, paddy field and wetland were measured. Using the method of redundancy analysis (RDA), the relationship between the distribution of calcium and soil physical and chemical indexes of 5 kinds of land use was analyzed. The results showed that in 5 types of land use, the calcium content of bare land and dry land was low, and the calcium content of woodland, paddy field and wetland was at a higher level. The spatial distribution of total calcium and 4 calcium forms was consistent and all showed forest land > paddy field > wetland > dry land > bare land. The order of calcium content in each type of land use was acid extractable calcium > reducible calcium > oxidized calcium > residual calcium. In general, the content of calcium was changed by the change of land use type, and the calcium content showed significant difference in soil depth, and was obvious in the surface enrichment. The soil in the basin was sandy soil, which was mostly neutral or slightly acidic. The content of organic matter was abundant and the phosphine level was higher. Redundant analysis showed that pH was the most important soil physicochemical index influencing the distribution of calcium content in different land use types.