Abstract: In the study, the culture conditions and sporulation characteristics of 2 entomopathogenic fungi (NH512, Y512) of Cephlica chuxiongica were studied, and the effects of temperature, light and UV mutagenesis on the growth and sporulation of fungi were analyzed. The results showed that the optimum temperature for mycelial growth of the strain NH512 is 25 ℃, in which the average colony diameter is 48.15 mm on the 15 day. The optimum temperature for mycelial growth of the strain Y512 is 20 ℃, in which the average colony diameter is 64.20 mm on the 15 day. Under the 25 ℃ condition of cultivation on the 15 day, the accumulation of spores of the strains NH512 and Y512 are maximum, which are 9.184 × 10⁷/cm² and 3.091 × 10⁷/cm², respectively. Both the alternating conditions of dark and light for 12 hours can promote the growth of 2 strains, and the average colony diameter are the maximum on the 15 day, which are 40.10 mm and 44.40 mm, respectively. No light condition is conducive to produce spores for NH512, and the condition of light and dark 12 hours alternatives can promote spores for Y512, and the accumulation of spores are 9.184 × 10⁷/cm² and 9.038 × 10⁷/cm² for both strains on the 15 day. UV mutagenesis can induce mycelium growth of NH512, but inhibite Y512. UV mutagenesis can inhibite NH512 to produce spores, but induce to produce spores for Y512 in which the average accumulation of spores are 15.69 × 10⁷/cm².