Abstract: Rosa chinensis was treated with tenuazonic acid (TeA) toxin of Alternaria tenuissima. To determine the content of protein, water, soluble sugar and elements in treated and above and below treatment site leaves of R.chinensis after 2, 6, 10, 14, 20 days. The methods are: Kjeldahl method, drying method, anthrone colorimetric method and inductively coupled plasma emission spectrometer (ICP-OES). The findings indicated that compared with healthy leaves, the contents of protein, water and soluble sugar in leaves treated with TeA decreased significantly. The change of protein content is complex, after 6 days treatment, the content of protein increased significantly, then it decreases with the increase of treated time. Protein content decreased from 208.41 mg/g to 145.09 mg/g; Moisture content decreased from 77.36% to 69.56%, dropped by 7.79%; Soluble sugar content decreased from 12.58 mg/g to 8.45 mg/g. After treated by the A.tenuissima toxin TeA, the content of mineral elements in rose leaves have changed, Ca, Mg, K, S, P, Zn et al decreased, but Al, Cu, Mn, Na et al increased. The toxin TeA treatment has a spatial effect, which affects the nutrient above and below the treated leaves.