Abstract: A randomized block experiment was conducted to study the effects of 8 kinds of nursery substrates on the rooting and growth of Alnus nepalensis after transplanting.Results showed that different seedling substrates significantly affected the rooting rate of cuttings.The rooting rate of T₁ and T₅ were significantly higher than those of other treatments, with a maximum of 34.44% for T₁ while there was no significant effect on the number of rooting of cuttings.The 8 kinds of nursery substrates significantly affected the new leaf germination, seedling height, and root growth 8 after transplanting, but the survival rate after transplanting was not significant, and T₁ was the most favorable for the growth after transplantation.The eight basic fertilizers significantly affected the survival rate, the new leaf germination, the seedling height, and the main root growth, and T_b was selected as the optimal base fertilizer.