油茶转录组测序与SSR特征分析

Transcriptome Sequencing and Analysis of SSR Characteristics of Camellia oleifera

摘要: 以油茶转录组测序数据为基础，利用MISA软件对SSR进行搜索，并对SSR特征进行分析评价。结果表明：油茶转录组中发现104 515个SSR，分布在80 724条Unigene中，分布密度为1/1.48 kb，平均出现频率为33.58%。主要的核酸重复类型是单核苷酸和二核苷酸，A/T和AG/CT是优势重复基元，重复比例最高的是10次。基序长度主要分布在12~20 bp之间，占总数的51.71%。油茶转录组SSR位点具有显著的数量优势、丰富的类型和较好的多态性潜力。

Abstract: With the sequencing data of Camellia oleifera as the research base, the SSR was searched through MISA software, and the SSR characteristics were analyzed and evaluated. The results showed that a total of 104 515 SSR were found and distributed in 80 724 Unigenes. The distribution density and the average frequency of occurrence were 1/1.48 kb and 33.58%, respectively. MNRs and DNRs were the major nucleic acid repeat types. A/T and AG/CT were the dominant repetitive bases. The proportion of the 10 repeats was the highest. The motifs′ length was concentrated at 12-20 bp, which was accounted for 51.71%. The SSR in the transcriptome of C.oleifera have significant quantitative advantages, rich types and good polymorphism potential.