Abstract: Three arbuscular mycorrhizal fungi, including Glomus mosseae, G.etunicatum and G.intraradensis, were selected to study the effect on the growth and drought resistance of Zenia insignis through mycorrhizal inoculation and potted culture with controlling soil water content. The results show that the rate of infection from G.mosseae is the highest (96.7%). The inoculation of 3 species arbuscular mycorrhizal fungi could all significantly increase the plant height, ground diameter, biomass accumulation and chlorophyll biosynthesis. The plant height growth of G.mosseae, G.intraradensis and G.etunicatum seedlings increased by 19%, 4.3% and 2.3%, the ground diameter increased by 18.6%、4.3% and 6.1%, and the chlorophyll content increased by 37.5%、11.2% and 10.8% respectively. Through the drought stress experiment, it is concluded that the relative water content and the soluble sugar content of mycorrhizal seedlings is significantly higher than that of CK, while the relative conductivity and malonaldehyde content of the mycorrhizal seedlings are significantly lower than that of CK, indicating that there is relatively little dehydrated behaviors of leaves of mycorrhizal seedlings, and the degree of damage on the cytomembrane is less, and more osmotic adjustment substances is produced at the same time. And the inoculation of arbuscular mycorrhizae could increase the photosynthetic capacity and water use efficiency, decrease the damage degree of ΦPSII reaction center, enhancing the efficiency of solar energy utilization and the drought-resistant ability of seedlings, among which the G.mossea has the best performance in drought resisting.