Abstract: Polyphenols were extracted from the seeds of Jatropha curcas, and the content of polyphenols was used as an index. The effects of acetone concentration, temperature, solid-liquid ratio and time on the extraction rate of polyphenols were analyzed by orthogonal experiment. The orthogonal experiment was used to explore the optimization process of extracting polyphenols from the seeds of J. curcas. Results show that the most suitable condition for the extraction of polyphenols is at acetone concentration 70%, extraction time 110 min. Under the optimum conditions, the polyphenols yield is 2.078 mg/g. The results of orthogonal experiment, the sequence in accordance with the merits of the extraction effect are ratio of solid-liquid > acetone concentration > extraction time > extraction temperature. The effects of liquid ratio, acetone concentration, time and temperature on the extraction of J. curcas kernel polyphenol are significantly different.