Abstract: Using Caragana korshinskii as test material, the L₁₆（4⁵） orthogonal test design was used to establish the polyploid induction system. The colchicine concentration, cytokinin concentration, radicle length and colchicine induction method were set. By observing the apical morphological index and microscopic comparison of chromosome number, a seed of induced polyploid C. korshinskii seeds was screened and verified. The best combination of treatments to create a new mutagenesis system. Results show that 0.02% colchicine, 0.01% cytokinin, 2 mm radicle length and embedding method are used to treat C. korshinskii radicle, and the induction effect is the best, and the induction rate is 56%.The concentration of cytokinin within 0.01% can increase the induction rate of radicle radicle, and the concentration of cytokinin from 0.01% to 0.03% can inhibit the induction.