Abstract: The Populus euramericana 'Bellotto' was used as the acceptor material, the genetic transformation of Vitis amurensis CBF3 gene was carried out by using Agrobacterium tumefaciens mediated blisk method. The effects of preculture time, bacterial concentration, infection time and co-culture time on transformation efficiency were studied, and the obtained resistant plants were detected by PCR. Results show that the suitable preculture time is 3 d, the OD₆₀₀ of suitable bacterial concentration is 0.3−0.4, the suitable infection time is 2−4 min and the suitable co-cultivation time is 3 d for genetic transformation process of the P. euramericana 'Bellotto'. After PCR identification of transformants, 4 positive transgenic plants are obtained, which preliminarily indicated that V. amurensis VaCBF3 gene is introduced into the P. euramericana 'Bellotto'. The conversion system has been successfully established.