Abstract: Scientific sampling plots investigation is an important premise for forest biomass estimation using remote sensing methods, and it is also the basis for improving the accuracy of biomass estimation. Through reviewing the methods of remote sensing estimation of sample surveys in the field of forest biomass research at home and abroad, and inductively analyzing them. It can be seen that the survey method varies according to different remote sensing data sources, research areas and survey objects, and involves the number of plots, plot layout, plot shape and size, plot survey factors and content. This led to the proposal of a sample survey to reduce the uncertainty of estimating forest biomass using remote sensing in the future.