紫果西番莲愈伤组织诱导分化及不定芽增殖研究

Induction and Differentiation of Callus and Propagation of Adventitious Buds in Passiflora caerulea

摘要: 以紫果西番莲离体培养的子叶为实验材料，对其愈伤组织进行诱导、分化及不定芽增殖。结果表明：在愈伤组织诱导中，以MS培养基+2,4-D 1.0 mg/L+TDZ 0.5 mg/L+琼脂3.8 g/L+蔗糖30 g/L的诱导效果最好，其诱导率为73.33%。在愈伤组织不定芽诱导中，以MS培养基+2,4-D 0.5 mg/L+TDZ 2.0 mg/L+琼脂3.8 g/L+蔗糖30 g/L的诱导率最佳，为46.67%。对不定芽继续进行增殖培养，以MS培养基+6-BA 1.0 mg/L+IAA 0.3 mg/L+琼脂4.0 g/L+蔗糖30 g/L最佳，增殖倍数为2.97。从而建立了西番莲植株再生体系，可进行优良材料的离体快繁。

Abstract: Cotyledons of Passiflora caerulea were used as experimental materials to induce callus, differentiation and adventitious buds proliferation. The results showed that the callus induction medium using MS+2,4-D 1.0 mg/L+TDZ 0.5 mg/L+Agar 3.8 g/L+Sucrose 30 g/L had the highest rate of callus induction, and the induction rate was 73.33%. The optimum medium for inducing buds was MS+2,4-D 0.5 mg/L+TDZ 2.0 mg/L+Agar 3.8 g/L+Sucrose 30 g/L, the induction rate reached 46.67%.The optimum medium for further culturing adventitious buds was MS medium+6-BA 1.0 mg/L+IAA 0.3 mg/L+Agar 4.0 g/L+Sucrose 30 g/L, the multiplication multiple was 2.97. The established plant regeneration system could provide technical support for rapid propagation in vitro, ploidy breeding and genetic transformation of P. caerulea.