华山松SRAP引物的筛选与验证

Selection and Validation of SRAP Primers in Pinus armandii

摘要: 为筛选华山松SRAP遗传多样性引物，以华山松无性系种子园6个种源的30株优良无性系幼嫩无病害针叶为实验材料，基于SRAP标记技术，选用10条正向引物、10条反向引物，随机组合成100个SRAP引物组合，对华山松DNA进行PCR扩增。结果表明：实验共筛选获得15对多态性较为丰富并且具有可重复性的有效引物；15对引物共扩增出3 767条DNA条带，其中，多态性条带有2 448条，占总条带数的64.99%，平均多态性比率为64.28%。所得15对SRAP引物适合作为华山松遗传多样性分析。

Abstract: In order to screen primers for SRAP genetic diversity of Pinus armandii, young disease-free needles from 30 superior clones from 6 provenances of P. armandii clonal seed orchard were used as experimental materials. Based on SRAP marker technique, P. armandii DNA were amplified with 100 SRAP primers combinations combined randomly with 10 forward and 10 reverse primers. It was found that a total of 15 pairs of effective primers with rich polymorphism and high repeatability were screened. A total of 3 767 DNA bands were amplified by 15 pairs of primers. Among them, 2 448 polymorphic bands accounted for 64.99% of the total bands, with an average polymorphic rate of 64.28%. The 15 pairs of SRAP primers obtained from the experiment were suitable for the genetic diversity analysis of P. armandii.