Abstract: Trichoderma virens Gv29-8 is one of the important biocontrol antagonists that have completed genome-wide sequencing. In order to predict and analyze the physicochemical properties of carbohydrate-active enzymes protein (CAZymes), 185 CAZymes proteins sequence in the T. virens Gv29-8 obtained in the previous period were the objects of the research, and the physicochemical properties were predicted and analyzed. The results showed that nearly two-thirds proteins theoretical isoelectric points (PI) is less than 6, which are acidic protein. At the same time, 10% random sampling analysis of the above proteins by the random number software, and it was found that the CAZymes proteins of different theoretical isoelectric points categories have different physicochemical properties such as molecular formula, atomic number, half-life, instability coefficient, aliphatic amino acid index and total average hydrophilicity. Among them, 26.67% of the proteins belong to unstable proteins and 13.33% of the proteins belong to hydrophilic proteins.