辣木茎叶营养成分及多酚提取物体外抗氧化研究

Analyses of Nutritional Components and Antioxidant Activity of Polyphenol Extracts from Stems and Leaves of Moringa oleifera

  • 摘要: 以辣木茎叶为原料,分析了辣木茎叶中粗蛋白、粗脂肪、粗纤维、粗灰分、无氮浸出物、钙、总磷及多酚含量,并提取了辣木茎叶多酚提取物,以DPPH自由基、ABTS自由基、羟自由基清除率以及金属螯合能力等指标评价辣木茎叶多酚提取物体外抗氧化能力。结果表明:辣木茎叶中粗蛋白含量为(17.98±0.87)%,粗脂肪含量为(2.71±0.17)%,粗纤维含量为(27.74±0.02)%,无氮浸出物含量为(35.56±0.96)%,粗灰分含量为(9.01±0.15)%,钙含量为(1.76±0.02)%,总磷含量为(0.62±0.01)%,多酚含量为(4.01±0.01)%。辣木茎叶多酚有较强的清除DPPH自由基的能力,浓度0.50 mg/mL时清除能力最强为91.08%,对羟基自由基的清除能力优于BHT,浓度1.25 mg/mL时,辣木茎叶多酚和BHT对羟自由基的清除率分别为97.50%和13.87%;辣木茎叶多酚对ABTS自由基表也现出了较强的清除能力,浓度1.25 mg/mL时,辣木茎叶多酚和BHT对ABTS自由基清除率分别为97.80%和99.18%;辣木茎叶多酚也具有一定的金属螯合能力,浓度在0.75 mg/mL时,辣木茎叶多酚金属螯合能力达到78.99%,而EDTA的金属螯合能力最高98.03%。

     

    Abstract: The crude protein, ether extract, crude fiber, crude ash, nitrogen-free extract, calcium, total phosphorus and polyphenols in the stems and leaves of Moringa oleifera were analyzed. The antioxidant capacity of M. oleifera stems-leaves polyphenol extract were evaluated by DPPH, ABTS free radical scavenging, hydroxyl radical and ferrous reductive capacity. The results showed that the crude protein, ether extract, crude fiber, nitrogen-free extract, crude ash, calcium, total phosphorus and polyphenols were (17.98±0.87)%, (2.71±0.17)%, (27.74±0.02)%, (35.56±0.96)%, (9.01±0.15)%, (1.76±0.02)%, (0.62±0.01)%, and (4.01±0.01)% respectively. The polyphenols from the stems-leaves of M. oleifera had a strong ability to scavenge DPPH free radicals, the strongest scavenging ability was 91.08% at the concentration of 0.5 mg·mL−1. The scavenging capacity of hydroxyl radicals was better than that of BHT. The scavenging rates of polyphenols from stems-leaves of M. oleifera and BHT were 97.50% and 13.87% respectively at polyphenol concentration 1.25 mg·mL−1. The ABTS free radical scavenging activity of polyphenols M. oleifera stems-leaves was also strong. When the polyphenols concentration was 1.25 mg·mL−1, the ABTS free radical scavenging rates of M. oleifera stems-leaves polyphenols and BHT were 97.80% and 99.18%, respectively. In addition, polyphenols from the stems-leaves of M. oleifera also have certain metal chelating ability. When the concentration of polyphenols in the stems-leaves of M. oleifera was 0.75 mg·mL−1, the metal chelating ability of polyphenols from the stems-leaves of M. oleifera and EDTA was 78.99% and 98.03%, respectively.

     

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