Abstract: In the study, the vine of pueraria lobata was used as the material, and the randomized block design was used to study the effects of ABT1 concentration, treatment time, cutting age and the length of cuttings retained under internodes on rooting characteristics. The findings indicated that the rooting characteristics of cuttings of different ages of P. lobata vine were significantly different, the rooting rate of 0.5-year-old vine cuttings was the highest, reaching 91.77%, and the average rooting number and root length of 1.5-year-old cuttings were the largest. The low concentration of ABT1 can promote the rooting of cuttings. With the increase of ABT1 concentration and the extension of treatment time, the rooting characteristics of wild P. lobata vine cuttings show a downward trend. The rooting rate, average rooting number, average root length and other rooting characteristics of 1.5-year-old wild P. lobata vine cuttings treated with 1.0×10⁻⁴ g/cm³ ABT1 are the best, which are 87.07%, 9.28 strips per plant, (3.94±1.28) cm per plant respectively. The vine cuttings of P. lobata are mainly of the skin rooting type, and the internodes are the easiest to root. The roots are mostly located on the back and internodes of vine cuttings.