Abstract: In this study, 3 investigations on species resources were carried out in Wanfeng Mountain Nature Reserve in 2018 by means of typical sampling plots and typical sampling transect lines. Based on the field investigation data and related references, the flora characteristics of seed plants in the nature reserve were analyzed in this paper. Results showed that there were 135 families, 573 genera, 964 species of seed plants in Wanfeng Mountain Nature Reserve, proportions of families, genus and species were different from Xianggelila County, but similar to the Ailao Mountain National Nature Reserve. With 90.77% of genera contained 1-5 species, that meant highly diversity at the level of species. At the family level, the tropic elements accounted for the highest proportion of 48.89% with 32.59% of the pantropic elements in Wanfeng Mountain. At the genus level, the temperate element was 41.53% only next to the percentage of 50.44% of tropic elements, showed clearly the flora transition from tropical to temperate. On the endemic element, there were only 2 endemic families to East Asian and 13 endemic genera to China in the nature reserve. However, endemism of the nature reserve was obviously lower compared with central and northwest Yunnan. Comparing with other compared areas, the flora of Wanfeng Mountain Nature Reserve was similar to Maolan Nature Reserve and Ailao Mountain National Nature Reserve, and was quite different from Guangxi Province and Xianggelila County.