4个无性系杉木心材挥发性成分的GC−MS分析

GC−MS Analysis of Volatile Components from the Heartwoods of 4 Cunninghamia lanceolata Clones

  • 摘要: 为明确不同无性系杉木心材挥发性成分的组成特点和含量差异,以浙江开化杉木无性系为研究对象,采用超临界CO2流体萃取技术提取各心材挥发油,运用GC−MS联用技术对挥发性成分的组成特点和含量进行分析。结果表明:1993年种植的开化3号和1998年开化3号、开化13号、F24x那1−1和大坝8号的挥发油得率分别为1.33%、1.34%、1.26%、1.04%和1.45%,其中大坝8号的提取率最高;从4个无性系5个样品挥发油中共鉴定出71种挥发性成分,包括萜烯类53种、萜醇类12种、萜烯酯类4种和其他2种,其中共有成分有31种;从1993年种植开化3号、1998年开化3号、开化13号、F24x那1−1和大坝8号中分别鉴定出48种、48种、52种、52种和50种挥发性成分,主要成分为柏木醇、α−可巴烯、β−石竹烯和泪杉醇等,其中柏木醇为5个样品中含量最高的化学成分;萜烯类和萜醇类成分相对含量较高,是杉木特殊香气的主要来源,但在不同无性系杉木心材中的组成特点和含量存在差异;通过主成分分析,1993年开化3号、1998年开化3号和开化13号成分较为相似,聚为一类,而1998年大坝8号和F24x那1−1分别与开化系列无性系心材挥发性成分的组成差异较大,各自聚为一类。

     

    Abstract: The volatile oils in the heartwoods of Cunninghamia lanceolata clones collected from Kaihua County, Zhejiang Province were extracted and analyzed to clarify the composition characteristics and content differences of volatile components in different clones. The carbon dioxide supercritical fluid extraction method was used to extract volatile oils, which were analyzed by gas chromatography-mass spectrometry (GC−MS), respectively. It was found that the yields of volatile oils from the heartwoods of 'Kaihua 3', 'Kaihua 13', 'F24x Na1−1' and 'Daba 8' clones planted in 1998, and 'Kaihua 3' clone planted in 1993 were 1.34%, 1.26%, 1.04%, 1.45% and 1.33%, respectively. Among them, the yield of volatile oil in 'Daba 8' heartwood was the highest. A total of 71 volatile components including 53 kinds of terpenes, 12 kinds of terpene alcohols, 4 kinds of terpene esters, and 2 kinds of the others were identified from the 5 samples of 4 clones, and the 31 compounds are common ones. Among 'Kaihua 3' planted in 1993, 'Kaihua 3', 'Kaihua 13', 'F24x Na1−1' and 'Daba 8' planted in 1998, 48, 48, 52, 52 and 50 volatile components were identified, respectively. The main components in 5 samples were cedarol, α−cobaene, β−caryophyllene and manool, and the content of cedrol was the highest in all samples. The terpenes and terpene alcohols with the higher contents should be the prime fragrance source of the C. lanceolata, but the some differences on the composition and content differences of volatile components in different clones. According to the results from principal component analysis, the 'Kaihua 3' planted in 1993, 'Kaihua 3' and 'Kaihua 13' planted in 1998 were grouped in the same category due to the similarity of the composition and contents of volatile components, and the 'F24x Na1−1' and 'Daba 8' were divided into the other 2 different categories.

     

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