Abstract: In this study, tobacco mosaic virus (TMV) was used as a model virus, and 27 plant ethanol extracts were screened by using half-leaf method and leaf disk method. According to the result, 12 plant extracts showed curative effects on TMV infection at a concentration of 1 mg/mL. Among them, Ageratum conyzoides is the most effective with inhibition rate of 53.06% relative to that of positive control Ningnanmycin (57.33%). Eight plant extracts play significant protective effects in TMV infection, the most effective is Coriaria nepalensis with a inhibition rate of 53.64%, followed by Parthenium hysterophorus and Artemisia palustris with inhibition rates of 46.51% and 36.63%, respectively. Nine plants exert inactivation effects on TMV, Crassocephalum crepidioides, Urtica fissa and Ageratum conyzoides have favorable inactivation activities, and the inhibition rates are 62.20%, 54.92% and 51.75%, respectively. Twenty-two extracts showed anti-TMV proliferation activity superior to or equivalent to Ningnanmycin. The concentration of TMV detected in Debregeasia orientalis and Conyza bonariensis are relative to that of the health tobacco, indicating the most significant anti-TMV proliferation activities.