杉木连栽地土壤的细菌群落结构与其特性的关系研究

Analysis of the Soil Bacterial Community Structure and Its Relationship with Soil Properties in Different Successive Rotation Cunninghamia lanceolata Plantations

  • 摘要: 探究不同连栽代数杉木人工林土壤细菌群落结构的多样性变化与土壤物理化学性质的相关性。在测定不同连栽代数杉木人工林土壤理化性质的基础上,利用高通量测序技术,测定土壤细菌数量与群落结构的多样性,并分析土壤细菌数量、群落结构的多样性与土壤理化性质间的相关性。结果表明:随着连栽代数的增加,杉木人工林土壤容重呈上升趋势,而土壤pH、全碳、全氮、总孔隙度体积等理化性质均呈下降趋势。不同连栽代数杉木人工林土壤细菌的优势门类为酸杆菌门、变形菌门、浮霉菌门、绿弯菌门;主要纲类有DA052、酸杆菌纲、α-变形菌纲、浮霉菌纲;优势目类为DA052 uncultured bacterium、酸杆菌目、红螺旋菌目、根瘤菌目、浮霉菌目。随着连栽代数增加,酸杆菌门、DA052纲相对丰度逐渐降低,而变形菌门、酸杆菌目、红螺旋菌目、根瘤菌目相对丰度则随连栽代数增加呈上升趋势。土壤细菌群落的群落指数Ace和群落指数Chao1均以1代杉木人工林为最高;土壤TK、TC、TN、TP、pH、毛管孔隙体积、含水量、土壤容重对主要细菌门、纲、目类相对丰度作用较大。群落指数Ace、群落指数Chao1、群落指数Simpson、群落指数Shannon分别与土壤容重、TP、TN、TC、含水量、非毛管孔隙体积、总孔隙度体积呈显著的相关性。

     

    Abstract: In order to explore the diversity of community structure of soil bacteria in different algebras of Cunninghamia lanceolata plantation and its correlation with soil physical and chemical properties. On the basis of determining the physical and chemical properties of different algebraic C. lanceolata soils, high-throughput sequencing technology was used to determine the number of soil bacteria and the diversity of community structure, and the correlation among the number of soil bacteria, the diversity of community structure and the physical and chemical properties of soil was analyzed. The results showed that the physical and chemical properties of soil pH, total carbon, total nitrogen, and total porosity volume showed a downward trend, except for soil bulk density, as the successive algebras increase. The dominant species of soil bacteria in different algebraic C. lanceolata plantations are Acidobacteria, Proteobacteria, Planctomycetes, and Chloroflexi at the phylum classification level. The main dominant species are DA052, Acidobacteria, α-Proteobacteria, and Planctomycetes at the class classification level. The main dominant species at the order classification level are DA052 uncultured bacterium, Acidobacteriales, Solirubrobacterales, Rhizobiales, and Planctomycetales. With the increase of crotations of continuous planting, the relative abundance of Acidobacteria and DA052 decreased gradually, while the relative abundance of Proteobacteria, Acidobacteria, Solirubrobacterales and Rhizobia increased gradually. The community index Ace of the soil bacterial community and the community index Chao1 were the highest in the 1st generation C. lanceolata plantation. Soil TK, TC, TN, TP, pH, capillary pore volume, water content, and soil bulk density have a greater effect on the relative abundance of major bacteria bacterial groups are at different levels of classification. The community index Ace, community index Chao1, community index Simpson, and community index Shannon were significantly correlated with soil bulk density, TP, TN, TC, water content, non-capillary pore volume, and total porosity volume.

     

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