Abstract: To screen the endophytic fungi with antifungal effect, the tissue isolation and confrontation culture were used to study the diversity of endophytic fungi in the young fruit of Vaccinium ashei and their antifungal effects on 6 kinds of plant pathogenic fungi. The results showed that 25 endophytic fungi were isolated from the young fruit of V. ashei, and the 25 strains were divided into 12 genera and 19 species by blastn analysis using rDNA−ITS sequence, with similarity of 97% −100%. Among them, there were 9 genera, 14 species and 20 strains of Ascomycota. Basidiomycota have 5 species and 3 genera. Diaporthe are the dominant genus of Ascomycota, accounting for 25% of the Ascomycota. The Shannon−Wiener diversity index H, Pielou index J and Margalef index R were 2.2870, 0.9203 and 3.4173 respectively. Among the 25 strains of endophytic fungi in the young fruit of V. ashei, the inhibition rate of Schizophyllum strain G6 against 5 kinds of pathogenic fungi was more than 40%, and that of G18 against 4 kinds of pathogenic fungi was more than 45%, showing good inhibition effect.