Abstract: In order to study the rooting effect of different plant growth regulators on shoot cuttings of Myricaria squamosa, one-year-old cuttings of M. squamosa in the same year were selected as the research object, adopted completely randomized design and the cuttings were treated with 3 kinds of plant growth regulators with different concentration gradients. The rooting rates and root related indexes under different treatments were compared and analyzed. The results showed that IBA could effectively improve the rooting rate and efficiency of M. squamosa in a short time, and the rooting rate under plant growth regulators was SA > IBA > CK > NAA. The number of roots of cuttings treated with NAA 50 mg/L was 16.68, which was significantly higher than that of other treatments. It can be seen that plant growth regulators have significant effect on softwood cuttings of M. squamosa, but the rooting effects of different growth regulators are different, and the concentration of IBA 50 mg/L has the best effect on the rooting of cuttings of M. squamosa.